| Project code |
Title |
Main con-tractor |
Total cost (£) |
2006/07 cost (£) |
Start date |
End date |
Primary aim |
Experimental details |
| BSE in cattle |
| SE0239 |
Investigation of genetic polymorphisms associated with BARB cases |
VLA |
267,802 |
0 |
2004 |
2006 |
To see if BSE cases in cattle Born After the Reinforced Ban (BARBs) differ from those associated with the food-borne epidemic |
Full DNA sequencing of the prion protein genes and 2-D gel electrophoretic analysis of the prion protein in approximately 100 BARB cases of BSE |
| SE1439 |
The relative transmissibility of sheep and cattle BSE to humans |
NPU, Roslin Institute |
1,153,100 |
123,400 |
2001 |
2006 |
To provide information on the relative species barrier to humans and bovines for BSE and scrapie |
Challenge of transgenic mice, which carry bovine or human prion protein genes, with sheep scrapie, sheep passaged BSE, and cattle BSE |
| SE1795 |
Investigation of TSE phenotype variation in British cattle |
VLA |
580,398 |
315,199 |
2005 |
2007 |
To search for any variation in British BSE cases using up-to-date methods |
Retrospective disease characterisation of brain tissue from infected animals identified collected throughout the BSE epidemic |
| SE1796 |
Atypical prion proteins in cattle |
VLA |
309,930 |
64,607 |
2005 |
2008 |
To see if any different types of BSE exist in GB |
Studies on the prion protein in suspected BSE cases which were not confirmed |
| SE1930 |
Further studies of the effect of oral inoculum dose on attack rate and incubation period of BSE in cattle |
VLA |
5,557,694 |
479,969 |
1997 |
2009 |
To determine the minimum oral dose for BSE in cattle |
Experimental challenges in cattle of single oral doses of 0.001g to 1g of BSE-infected brain |
| SE1941 |
Studies to examine the pathogenicity, phenotype and pathogenesis of endemic scrapie in cattle |
VLA |
4,221,050 |
461,331 |
1998 |
2008 |
To see if sheep scrapie can cause disease in cattle |
Intracerebral and oral challenges of cattle with various pools of sheep scrapie isolates |
| SE1942 |
The attack rate and phenotype of scrapie-like disease on transmission to cattle of scrapie |
VLA |
3,140,743 |
0 |
1997 |
2005 |
To see if sheep scrapie can cause disease in cattle |
Oral challenges of cattle with pools of raw and rendered sheep scrapie-infected brain homogenate |
| Animal by-products |
| Controls |
| SE0234 |
The effect of rendering on TSE agent strain type |
VLA |
235,115 |
15,000 |
2001 |
2007 |
To see if rendering changes the properties of BSE |
Mouse bioassay of the fractions of bovine BSE resulting from Carver-Greenfield rendering |
| SE0247 |
Evidence base to TSE control risk assessment |
DNV |
14,000 |
14,000 |
2006 |
2007 |
To identify any data gaps for future risk assessments |
Analysis of the quality of existing evidence that informs the EU TSE Roadmap |
| SE1425 |
Inactivation of TSE agents by a novel biorefinement system |
NPU, Roslin Institute |
112,100 |
0 |
1999 |
2008 |
To assess a novel inactivation method |
Measurement by mouse bioassay of the level of TSE infectivity remaining after BSE-spiked material is subjected to a "biolytic" process |
| SE1438 |
Characterisation of the effect of heat on TSE agent strains |
NPU, Roslin Institute |
523,000 |
0 |
2001 |
2007 |
To allow predictions of how TSE infectivity is inactivated by heat |
Bioassay of tissue from animals infected with heat-treated TSE strains |
| SE1797 |
Detection of presence of species-specific processed animal proteins in animal feed |
VLA |
140,661 |
11,885 |
2007 |
2009 |
To provide tests for necessary controls if amendment of the total feed ban is approved |
Development of suitable validated methods for the species specific detection and quantification of animal protein in compound feed |
| SE1798 |
Detection of presence of species-specific processed animal proteins in animal feed |
CSL |
90,928 |
9,862 |
2007 |
2009 |
To provide tests for necessary controls if amendment of the total feed ban is approved |
Development of a species-specific test based on the detection of animal muscle proteins by mass spectrometry |
| TSEs in the environment |
| SE1433 |
Studies on the environmental persistence of TSE infectivity |
NPU, Roslin Institute |
2,588,255 |
265,150 |
1999 |
2011 |
To provide indicative data for risk assessments of BSE in the environment |
A field lysimeter-based experiment measuring the persistence in, and migration of, prions from both a bolus of infectivity and a buried carcase |
| SE1856 |
Investigation of the sources of TSE infection for the lamb in the prenatal and perinatal period |
VLA |
616,726 |
166,906 |
2005 |
2008 |
To identify the route of transmission for BSE and scrapie in the perinatal period |
Detailed examination of tissues and other fluids from scrapie-infected ewes their lambs during pregnancy and in the perinatal period |
| SE1858 |
Biochemical and bioassay analysis of the persistence of TSE infectivity in soil |
ADAS |
616,659 |
0 |
2007 |
2011 |
To provide information on the presence bioavailability of soil-bound prion proteins |
Studies on the binding and bioavailability of PrPSc in soil |
| SE1859 |
Investigation of the infectivity of faeces outside the perinatal period in animals of known TSE status |
VLA |
67,787 |
0 |
2007 |
2008 |
To examine whether faeces may be a route of transmission for scrapie |
Development of a biochemical test for PrPSc in faeces |
| SE1845 |
Studies on the transmission of natural scrapie in an infected flock |
VLA |
1,308,285 |
207,210 |
2001 |
2008 |
To identify routes of environmental transmission for scrapie |
Exposure of scrapie-free sheep to different environments (lambing, pasture, sheds) in a heavily infected flock |
| Sheep and goat TSEs |
| Classical scrapie |
| SE1432 |
The susceptibility of New Zealand sheep to TSE infection and linkage with PrP genotypes |
NPU, Roslin Institute |
1,331,440 |
1998 |
1998 |
2007 |
To establish whether New Zealand sheep have the same susceptibility to scrapie and BSE as UK sheep |
Challenges of New Zealand sheep of various genotypes with BSE and scrapie |
| SE1442 |
Goat BSE: Proposal for improvement of goat TSE discriminative diagnosis and susceptibility based assessment of BSE infectivity in goat milk and meat |
NPU, Roslin Institute |
302,190 |
2006 |
2006 |
2010 |
To establish the frequencies associated with allelic variants at PRNP in goats |
Sequencing of the prion protein gene in GB flocks, including scrapie-affected animals and susceptibility studies (this project is part of a large EU collaborative project) |
| SE1758 |
To maintain a nucleus flock of scrapie resistant Swaledale sheep and use for research into PrP genotype |
ADAS |
535,903 |
35,288 |
1999 |
2009 |
To identify any relationships between prion protein genotype and performance traits in Swaledale sheep |
Analysis of lamb crop statistics and susceptibility studies |
| SE1834 |
The role of the pre-implantation embryo in the vertical transmission of natural scrapie infection |
SAC |
988,502 |
0 |
1998 |
2006 |
To see if pre-implantation embryos are a transmission route for scrapie |
Transfer of embryos from natural scrapie-infected donor ewes to scrapie-free recipients |
| SE1843 |
Maternal transmission of scrapie in sheep |
NPU, Roslin Institute |
2,214,874 |
65,694 |
2001 |
2010 |
To examine the role of prion protein genotype on maternal transmission of scrapie |
Studies on lambs born naturally to experimentally-challenged ewes at late stages of disease |
| SE1845 |
Studies on the transmission of natural scrapie in an infected flock |
VLA |
1,308,285 |
207,210 |
2001 |
2008 |
To identify routes of environmental transmission for scrapie |
Exposure of scrapie-free sheep to different environments (lambing, pasture, sheds) in a heavily infected flock |
| SE1851 |
Susceptibility of ARR/ARR Sheep to scrapie challenge by intercerebral challenge |
VLA |
132,341 |
44,147 |
2006 |
2008 |
To provide comparative data for BSE transmission studies in ARR/ARR sheep |
Intracerebral challenges of ARR/ARR with classical scrapie |
| SE1852 |
Carrier state: do sheep of resistant PrP genotypes become sub-clinically infected when exposed to scrapie? (part 2) |
NPU, Roslin Institute |
192,834 |
47,001 |
2006 |
2010 |
To see if ARR/ARR sheep can be carriers for classical scrapie |
Testing of tissues from ARR/ARR sheep exposed to classical scrapie by bioassay in VRQ/VRQ sheep |
| SE1853 |
Carrier state: do sheep of resistant PrP genotypes become sub-clinically infected when exposed to scrapie (part 1) |
NPU, Roslin Institute |
905,164 |
293,568 |
2006 |
2009 |
To see if ARR/ARR sheep can be carriers for classical scrapie |
Testing of tissues from ARR/ARR sheep exposed to classical scrapie by bioassay in transgenic mice carrying sheep prion protein genes |
| SE1854 |
To test for the potential presence of scrapie PrPSc and any associated infectivity in naturally exposed and/or experimentally challenged ARR/ARR sheep using biochemical and transgenic techniques |
VLA |
779,835 |
198,132 |
2005 |
2008 |
To see if ARR/ARR sheep can be carriers for classical scrapie or BSE |
Testing of tissues from ARR/ARR sheep exposed to classical scrapie and BSE by sensitive biochemical tests and by bioassay in transgenic mice carrying sheep prion protein genes |
| SE1855 |
Investigation of the risk of transmission of scrapie in milk of sheep |
VLA |
1,213,859 |
38,823 |
2006 |
2013 |
To see if milk is a transmission route for sheep scrapie |
Bottle-feeding of milk and colostrum from individual scrapie-infected ewes to scrapie-free lambs |
| SE1856 |
Investigation of the sources of TSE infection for the lamb in the prenatal and perinatal period |
VLA |
616,726 |
166,906 |
2005 |
2008 |
To identify the route of transmission for BSE and scrapie in the perinatal period |
Detailed examination of tissues and other fluids from scrapie-infected ewes their lambs during pregnancy and in the perinatal period |
| SE1857 |
Investigation of sheep scrapie transmission via milk from the inflamed mammary gland |
University Hospital Zurich |
176,430 |
58,810 |
2005 |
2008 |
To see if the presence of viral mastitis affects the risk of scrapie transmission through milk |
Analysis of mammary gland tissues in scrapie, Maedi-visna virus (MVV) and scrapie and MVV-infected ewes and feeding of milk from these ewes to scrapie-free lambs |
| SE1950 |
Inter-breed, cross genotype transmission studies of ovine scrapie |
MRI |
2,330,410 |
319,301 |
2001 |
2009 |
To examine whether strains are stable in sheep of different breeds and genotypes |
Challenges of Cheviot VRQ/VRQ scrapie in Suffolk ARQ/ARQ sheep and vice versa |
| SE1953 |
A study of the pathogenesis and clinical signs of experimental scrapie in sheep |
VLA |
2,188,938 |
150,939 |
2001 |
2008 |
To describe the progression of scrapie disease in sheep |
Study of the clinical symptoms and disease pathogenesis in sheep experimentally challenged with a pool of scrapie-infected brains |
| SE1954 |
Studies on the pathogenesis of natural scrapie in an infected flock |
VLA |
909,003 |
0 |
2001 |
2006 |
To characterise natural scrapie in sheep |
Immunohistochemical studies of a range of tissues taken at preclinical stages from naturally-infected sheep of various breeds and genotypes |
| SE1955 |
Studies on the alimentary pathogenesis of scrapie infection |
MRI |
942,427 |
268,250 |
2003 |
2010 |
To define the routes of uptake of BSE and scrapie in sheep |
Studies on the distribution of scrapie and BSE in gut-associated lymphoid tissue after injection into gut loops |
| SE2004 |
Identification of PrP associated infectivity in blood and milk from sheep infected with TSE, toward a diagnostic test for live animals |
CEA France |
136,700 |
58,533 |
2005 |
2008 |
To develop a blood test for TSEs |
Measurement of scrapie infectivity in blood and milk and different time points after experimental challenge |
| Naturally occurring TSEs in sheep |
| SE1849 |
Strain characterisation of primary isolates of natural sheep scrapie |
VLA |
1,232,933 |
205,585 |
2005 |
2009 |
To catalogue the scrapie strains present in the British sheep |
Strain typing of field scrapie isolates by mouse bioassay |
| SE1919 |
Studies to identify possible homologies between TSEs |
VLA |
1,085,378 |
0 |
1995 |
2005 |
To see if BSE can be detected in GB sheep |
Strain typing field isolates of sheep scrapie in mice |
| SE1938 |
Strain typing of isolates of natural scrapie: correlations with host PrP genotype and pathological phenotype |
VLA |
2,729,710 |
0 |
1998 |
2006 |
To see if there is any influence of the sheep host on the scrapie strain type |
Strain characterisation in sheep and by mouse bioassay of naturally-occurring scrapie isolates from sheep carrying different genotypes on the same farms |
| Atypical scrapie |
| SE0240 |
Ovine PrP polymorphism: investigation of unclassified scrapie cases |
VLA |
317,693 |
0 |
2005 |
2006 |
To identify specific polymorphisms in the prion protein gene associated with atypical scrapie |
DNA sequencing of the prion protein gene in sheep carrying atypical scrapie infection |
| SE0248 |
A VLA audit of ovine and caprine formalin-fixed, paraffin-embedded tissues to assess suitability for a retrospective search for atypical scrapie |
VLA |
26,023 |
26,023 |
2006 |
2007 |
To establish the earliest date at which atypical scrapie can be identified in GB |
Retrospective analysis of archived tissues for the presence of atypical scrapie |
| SE0251 |
A search for UK atypical scrapie in archival samples and in classical scrapie resistant sheep |
NPU, Roslin Institute |
169,666 |
0 |
2007 |
2008 |
To establish the earliest date at which atypical scrapie can be identified in GB |
Retrospective analysis of archived tissues for the presence of atypical scrapie |
| SE1441 |
The relative transmissibility of atypical forms of scrapie to humans |
NPU, Roslin Institute |
700,685 |
125,370 |
2006 |
2010 |
To provide information on the relative species barrier to humans and bovines for atypical scrapie |
Challenge of transgenic mice, which carry bovine or human prion protein genes, with atypical scrapie, sheep BSE and classical scrapie |
| SE1789 |
Unclassified (anomalous) scrapie survey samples: biochemical and immunological characterisation |
VLA |
248,460 |
2004 |
2004 |
2007 |
To characterise the prion protein in atypical scrapie |
Biochemical studies, including proteinase K digestion kinetics on samples of atypical and classical scrapie |
| SE1847 |
Anomalous scrapie transmission to sheep |
VLA |
741,974 |
170,442 |
2004 |
2009 |
To see if atypical scrapie is transmissible from sheep to sheep |
Oral and intracerebral challenges of scrapie-free sheep with atypical scrapie |
| SE1850 |
Transmission of unclassified scrapie survey samples to conventional and transgenic mice |
VLA |
903,018 |
277,871 |
2004 |
2009 |
To see if atypical scrapie is a transmissible disease |
Challenges of transgenic mice carrying sheep prion protein genes with atypical scrapie [in collaboration with French and German laboratories] |
| Experimental BSE in sheep and goats |
| SE1424 |
The study of BSE in sheep and the possibility of its vertical transmission |
NPU, Roslin Institute |
1,134,659 |
25,127 |
1996 |
2009 |
To see if BSE can be transmitted from ewe to lamb |
Analysis of successive lamb crops born to experimentally ewes of various PrP genotypes |
| SE1428 |
Pathogenesis studies of experimental BSE in sheep |
NPU, Roslin Institute |
4,361,696 |
168,103 |
1997 |
2008 |
To define which sheep tissues would carry BSE infectivity |
Mouse bioassay analysis of distribution of BSE infectivity in tissues in orally challenged ARQ/ARQ sheep |
| SE1429 |
Characterisation (by transmission to mice) of BSE from experimentally infected sheep of different PrP genotypes |
NPU, Roslin Institute |
808,485 |
57,785 |
1998 |
2007 |
To characterise the behaviour of BSE in sheep under different conditions |
Analysis by mouse bioassay of BSE in sheep of different genotypes and scrapie status, and of mixed BSE/scrapie inocula |
| SE1432 |
The susceptibility of New Zealand sheep to TSE infection and linkage with PrP genotypes |
NPU, Roslin Institute |
1,331,440 |
0 |
1998 |
2007 |
To establish whether New Zealand sheep have the same susceptibility to scrapie and BSE as UK sheep |
Challenges of New Zealand sheep of various genotypes with BSE and scrapie |
| SE1435 |
Is the BSE strain phenotype stable on sub-passage in sheep |
NPU, Roslin Institute |
3,808,879 |
394,917 |
2000 |
2010 |
To see the BSE agent would be stable when circulating in sheep |
Serial passaging of BSE in Cheviot sheep |
| SE1436 |
Does scrapie prevent BSE from replicating and producing disease in a single host? |
NPU, Roslin Institute |
1,117,756 |
65,694 |
2001 |
2008 |
To examine the effect of a mixed infection of BSE and scrapie |
Analysis by mouse bioassay of the infection induced after challenges of sheep with both BSE and scrapie |
| SE1442 |
Goat BSE: Proposal for improvement of goat TSE discriminative diagnosis and susceptibility based assessment of BSE infectivity in goat milk and meat |
NPU, Roslin Institute |
302,190 |
80,433 |
2006 |
2010 |
To establish the frequencies associated with allelic variants at PRNP in goats |
Sequencing of the prion protein gene in GB flocks, including scrapie-affected animals and susceptibility studies (this project is part of a large EU collaborative project) |
| SE1842 |
Comparative titration of infectivity of BSE in sheep and mice |
MRI |
1,292,496 |
25,000 |
2000 |
2007 |
To enable estimation of infectivity in sheep by titration in mice |
Simultaneous titration of a primary sheep BSE inoculum in sheep and mice |
| SE1844 |
The effect of age at challenge on the subsequent development of BSE in sheep |
NPU, Roslin Institute |
1,684,102 |
290,699 |
2001 |
2010 |
To establish whether there is an effect of age on the susceptibility to BSE |
Challenge of AxQ/AxQ sheep with different doses of BSE at different ages |
| SE1846 |
Investigation of the effective minimum oral dose of BSE in PrP genotype susceptible sheep of different breeds |
VLA |
2,844,122 |
436,387 |
2002 |
2011 |
To determine the minimum oral dose for BSE in sheep |
Challenge of sheep of different breeds with 0.0005g–5g of cattle BSE |
| SE1929 |
Studies of experimental BSE in genetically susceptible sheep |
VLA |
7,281,868 |
282,217 |
1997 |
2013 |
To define which sheep tissues would carry BSE infectivity |
Analysis by mouse bioassay of distribution of BSE infectivity in tissues in orally challenged ARQ/ARQ, ARQ/ARR and ARR sheep |
| SE1945 |
Investigation of sheep to sheep passage on the BSE strain phenotype |
VLA |
4,997,483 |
937,075 |
1999 |
2016 |
To see if the BSE agent would be stable when circulating in sheep and to determine the minimum oral dose for sheep BSE |
Serial passaging of different doses of sheep BSE in sheep |
| SE1946 |
BSE in sheep: supply of tissues for further research |
VLA |
3,052,753 |
406,572 |
2001 |
2010 |
To see if BSE can sustain itself in an sheep flock |
Maintenance of experimentally infected ewes as a flock |
| SE1952 |
Studies on the immune system during infection of sheep with the scrapie and BSE agent |
MRI |
811,669 |
72,267 |
2001 |
2006 |
To establish the role of the immune system in scrapie pathogenesis |
Analysis of skin biopsies and lymph nodes taken from cannulated sheep that have been experimentally challenged |
| Flocks of known TSE status |
| SE0230 |
Development and maintenance of a flock of UK sheep naturally infected with scrapie |
VLA |
3,316,941 |
407,396 |
2000 |
2009 |
To provide a resource of natural scrapie-infected sheep, of different genotypes and breeds |
Maintenance of an established and diverse flock (the Ripley flock) endemically infected with different strains of scrapie, supplier of many sheep tissues for research |
| SE1758 |
To maintain a nucleus flock of scrapie resistant Swaledale sheep and use for research into PrP genotype |
ADAS |
535,903 |
35,632 |
1905 |
2009 |
To identify any relationships between prion protein genotype and performance traits in Swaledale sheep |
Analysis of lamb crop statistics and susceptibility studies |
| SE1931 |
Maintenance of a TSE-free sheep flock after importation from New Zealand |
VLA |
10,303,916 |
958,717 |
1997 |
2009 |
To provide a resource of scrapie free sheep, of different genotypes and breeds |
Maintenance of an imported breeding flock of NZ sheep |
| SE1949 |
The effect of tonsil biopsy on the rate and frequency of disease in 171 QQ susceptible Suffolk sheep |
MRI |
2,249,872 |
341,602 |
2001 |
2008 |
To see if tonsil biopsies affect the spread or onset of scrapie |
Analysis of the effect of single and multiple tonsil biopies in a naturally infected flock |
| Epidemiology/Modelling |
| SE0213 |
An epidemiological study of sheep scrapie to determine means of natural transmission |
VLA |
3,348,686 |
0 |
1995 |
2006 |
To identify risk factors for classical scrapie in flocks |
A formal epidemiological study to identify risk factors important in the maintenance of infection and disease in flocks. |
| SE0227 |
Investigation of flock level risk factors for scrapie |
VLA |
1,812,293 |
0 |
1999 |
2006 |
To identify risk factors for classical scrapie in flocks |
A case control study to identify risk factors for the introduction and maintenance of scrapie in sheep flocks |
| SE0236 |
Selective breeding on PrP genotype in the UK sheep flock: evaluating the consequences and deriving optimal strategies |
SAC |
1,869,155 |
461,321 |
2004 |
2007 |
To identify any potential effects of the National Scrapie Plan breeding programme on sheep traits |
Analysis of PrP genotype and traits of economic importance to the farmer in a wide range of commercial flocks (in collaboration with MLC, Roslin, University of Edinburgh and MLC) |
| SE0243 |
Analysis and design of scrapie surveillance strategies in Great Britain |
VLA |
561,851 |
335,060 |
2005 |
2008 |
To optimise surveillance strategies and ensure valid prevalence comparisons |
Modellling analysis of scrapie surveillance data (in collaboration with IAH, RVC and University of Oxford) |
| SE0244 |
Designing an abattoir survey to determine the PrP genotype profile of the slaughter lamb population in Great Britain |
VLA |
234,517 |
196,696 |
2006 |
2007 |
To design a survey that could be used to assess the National Scrapie Plan |
Sample design taking into account the relationship between breeds and abattoirs |
| SE0245 |
Ovine PrP gene sequences from sheep with complex genotypes |
NPU, Roslin Institute |
29,280 |
3,253 |
2005 |
2006 |
To confirm the absence of artefacts in defining complex genotypes in sheep |
DNA sequencing of the prion protein gene in sheep with complex genotypes |
| SE0246 |
Demographic predictors for scrapie-affected farms |
University of Oxford |
9,678 |
0 |
2005 |
2005 |
To explore the effect of sheep movements on the spread of scrapie |
Paired and case control comparisons of farms with and without scrapie using information from existing databases |
| SE0248 |
A VLA audit of ovine and caprine formalin-fixed, paraffin-embedded tissues to assess suitability for a retrospective search for atypical scrapie |
VLA |
26,023 |
2006 |
2006 |
2007 |
To establish the earliest date at which atypical scrapie can be identified in GB |
Retrospective analysis of archived tissues for the presence of atypical scrapie |
| SE0249 |
Assessing the design and efficacy of control strategies for classical scrapie which make use of live diagnostic tests |
IAH, Pirbright |
254,736 |
0 |
2007 |
2009 |
To advise on the efficient use of pre-clinical tests in sheep |
Modelling study using both within and between flock approaches |
| SE0250 |
Evaluating a live test for scrapie: epidemiology, economics and a national flock perspective |
University of Glasgow |
190,474 |
0 |
2007 |
2009 |
To advise on the efficient use of pre-clinical tests in sheep |
Modelling studies to include live animal testing in an existing model and to use network-based models |
| SE0251 |
A search for UK atypical scrapie in archival samples and in classical scrapie resistant sheep |
NPU, Roslin Institute |
169,666 |
2007 |
2007 |
2008 |
To establish the earliest date at which atypical scrapie can be identified in GB |
Retrospective analysis of archived tissues for the presence of atypical scrapie |
| TSE Diagnostics |
| Live animal tests |
| SE0249 |
Assessing the design and efficacy of control strategies for classical scrapie which make use of live diagnostic tests |
IAH, Pirbright |
254,736 |
0 |
2007 |
2009 |
To advise on the efficient use of pre-clinical tests in sheep |
Modelling study using both within and between flock approaches |
| SE0250 |
Evaluating a live test for scrapie: epidemiology, economics and a national flock perspective |
University of Glasgow |
190,474 |
0 |
2007 |
2009 |
To advise on the efficient use of pre-clinical tests in sheep |
Modelling studies to include live animal testing in an existing model and to use network-based models |
| SE1736 |
Experimental production of bovine tissues for validation of BSE diagnostic tests |
VLA |
5,769,882 |
0 |
1997 |
2006 |
To ensure bovine BSE-infected tissues are available for research and test validation |
Harvesting and storage of tissues from cattle experimentally infected with BSE at different time points after challenge |
| SE1773 |
Development of novel aptamer-based reagents and protocols for the diagnosis of TSEs |
University College London |
377,968 |
0 |
2002 |
2008 |
To assess a novel test method for TSEs |
Production and investigation of the use of aptamers to detect PrPSc |
| SE1775 |
Identification of new biomarkers for early natural TSE detection using genome-wide approach |
CIADC |
445,480 |
0 |
2002 |
2005 |
To identify new markers for scrapie in sheep |
Definition of expression profiles from scrapie-infected and scrapie-free sheep using microarray hybridisation |
| SE1780 |
Measurement of prion infectivity in blood during TSE disease |
University of Cambridge |
478,144 |
87,024 |
2003 |
2007 |
To determine if blood from scrapie-infected sheep contains TSE infectivity |
Bioassay of blood and lymphoid material in transgenic mice carrying bovine or ovine prion protein genes |
| SE1781 |
Generation of novel recombinant scFv antibodies to conformationally unique prion protein peptides |
Queens University Belfast |
249,573 |
0 |
2003 |
2005 |
To generate novel antibodies against the prion protein |
Generation of new antibodies against conformational features of PrP |
| SE1783 |
Examination of genes differentially expressed in immune cells following BSE challenge as a test for TSE infection |
Roslin Institute |
427,602 |
51,838 |
2003 |
2007 |
To identify new markers for BSE |
Definition of gene expression profiles using microarray hybridisation |
| SE1786 |
Collection of the biological resources for the identification of surrogate markers for BSE infection in cattle |
Roslin Institute |
117,069 |
20,000 |
2003 |
2008 |
To provide fractionated white blood cells for use in project SE1783 |
Fractionation of white blood cells from BSE-infected cattle taken at different time points after challenge |
| SE1788 |
Efficiency of rectal mucosa biopsies in the diagnosis of pre-clinical sheep scrapie |
VLA |
566,589 |
73,355 |
2004 |
2008 |
To provide a live animal test for sheep |
Immunohistochemical and biochemical analysis of samples of recto-anal mucosal associated lymphoid tissue (RAMALT) |
| SE1793 |
Disease-associated PrP detection in blood of scrapie and BSE infected sheep |
VLA |
358,975 |
113,338 |
2005 |
2008 |
To develop a blood test for TSEs |
Flow cytometry of blood cells of TSE-infected sheep, using PrPSc-selective antibody binding |
| SE1946 |
BSE in sheep: supply of tissues for further research |
VLA |
3,052,753 |
406,572 |
2001 |
2010 |
To see if BSE can sustain itself in an sheep flock |
Maintenance of experimentally infected ewes as a flock |
| SE2004 |
Identification of PrP associated infectivity in blood and milk from sheep infected with TSE, toward a diagnostic test for live animals |
CEA, France |
136,700 |
58,533 |
2005 |
2008 |
To develop a blood test for TSEs |
Measurement of scrapie infectivity in blood and milk and different time points after experimental challenge |
| SE2005 |
Assessment of candidate secreted surrogate biomarkers for early diagnosis of prion disease in farm animals |
University Hospital Zurich |
174,124 |
83,354 |
2005 |
2007 |
To assess two surrogate markers for TSEs |
Development of methods to detect bovine and ovine homologues of two secreted proteins highly expressed in human prion disease |
| SE2006 |
A rapid sensitive live animal blood test for sheep scrapie |
Adlyfe Inc, USA |
348,995 |
178,450 |
2005 |
2007 |
To develop a blood test for scrapie |
Further development of Adlyfe’s Misfolded Protein Diagnostic (MPD) assay |
| SE2008 |
Development of rapid pre-mortem tests for TSEs in sheep |
ADAS |
465,674 |
164,066 |
2005 |
2008 |
To develop a blood test for scrapie |
Assessment of combinations of sensitive methods for the detection of PrPSc in scrapie-infected sheep |
| SE2009 |
Detailed characterisation of novel phage antibodies that bind the amino terminus of the prion protein |
Reading University |
179,543 |
116,400 |
2006 |
2007 |
To develop new antibodies for the prion protein |
Characterisation of single chain antibodies raised against the N-terminus of the prion protein |
| SE2010 |
A blood test for TSEs |
VLA |
180,087 |
59,155 |
2005 |
2008 |
To develop a blood test for TSEs in sheep |
Application of IDEXX tests to detect PrPSc in sheep blood |
| SE2011 |
Enrichment and isolation of abnormal PrP from body fluids including blood, cerebrospinal fluid and urine |
VLA |
187,105 |
123,207 |
2005 |
2007 |
To develop a blood test for TSEs in sheep |
Identification of blood fractions with high levels of disease-associated PrP |
| SD0405 |
Conformational analysis of prion proteins: towards an ante-mortem test for BSE: an ion mobility/mass spectrometry approach |
Warwick University |
413,735 |
145,591 |
2005 |
2007 |
To develop a live animal test |
Computer modelling coupled with ion mobility and mass spectrometry |
| SD0426 |
Preparation of standard materials from cases of BSE and scrapie |
NIBSC |
131,068 |
47,449 |
2005 |
2006 |
To provide standardised tissues for test validation |
Preparation of multiple aliquots from BSE and scrapie infected tissues |
| TSE Strain typing |
| SE0241 |
Statistical analysis of strain typing data derived from sheep TSEs |
Risk Solutions |
224,956 |
0 |
2005 |
2006 |
To improve discrimination of TSE at first passage in mice |
Application of statistical methods based on cluster analysis of lesion profile data (in collaboration with NPU and VLA) |
| SE0242 |
The application of cluster analysis methods to the analysis of BSE strain typing data |
Risk Solutions |
39,914 |
0 |
2005 |
2005 |
To improve discrimination of TSE at first passage in mice |
Application of statistical methods based on cluster analysis of lesion profile data (in collaboration with NPU and VLA) |
| SE1440 |
Do strains of mouse adapted scrapie and BSE correlate with strains seen in the natural host? |
NPU, Roslin Institute |
1,260,900 |
0 |
2001 |
2007 |
To establish if BSE strain characteristics are affected by the species of prion protein in mice |
Alternate passaging of BSE through wild type mice and transgenic mice carrying bovine prion protein |
| SE1700 |
Multiplex approaches for biochemical TSE strain characterisation and differentiation |
VLA |
653,785 |
0 |
2007 |
2010 |
To develop a more rapid method of strain typing |
Development of multiplex immunoblotting by tagging existing antibodies with fluorescent dyes |
| SE1785 |
Fingerprinting sheep brain PrPSc by mass spectrometry to discriminate BSE and scrapie |
VLA |
569,010 |
8,000 |
2003 |
2007 |
To develop rapid methods of discriminating TSEs |
Mass spectrometry and 1-D and 2-D gel electrophoretic analysis of PrPSc in BSE and scrapie |
| SE1789 |
Unclassified (anomalous) scrapie survey samples: biochemical and immunological characterisation |
VLA |
248,460 |
69,273 |
2004 |
2007 |
To characterise the prion protein in atypical scrapie |
Biochemical studies, including proteinase K digestion kinetics on samples of atypical and classical scrapie |
| SE1792 |
Molecular strain-typing of animal TSE diseases |
VLA |
621,991 |
103,825 |
2004 |
2006 |
To develop biochemical methods of strain typing |
Development of strain-typing tests based on preferential proteolytic digestion |
| SE1795 |
Investigation of TSE phenotype variation in British cattle |
VLA |
580,398 |
315,199 |
2005 |
2007 |
To search for any variation in British BSE cases using up-to-date methods |
Retrospective disease characterisation of brain tissue from infected animals identified collected throughout the BSE epidemic |
| SE1796 |
Atypical prion proteins in cattle |
VLA |
309,930 |
64,607 |
2005 |
2008 |
To see if any different types of BSE exist in GB |
Studies on the prion protein in suspected BSE cases which were not confirmed |
| SE1848 |
Molecular transmission characteristics of ovine scrapie prion strains |
University of Cambridge |
1,035,191 |
272,441 |
2005 |
2009 |
To develop a biochemical test for strain typing |
Biological and biochemical characterisation of ovine scrapie strains, and the strains subsequently derived from them in mice (in collaboration with VLA) |
| SE1849 |
Strain characterisation of primary isolates of natural sheep scrapie |
VLA |
1,232,933 |
2005 |
2005 |
2009 |
To catalogue the scrapie strains present in the British sheep |
Strain typing of field scrapie isolate by mouse bioassay |
| SE1919 |
Studies to identify possible homologies between TSEs |
VLA |
1,085,378 |
0 |
1995 |
2005 |
To see if BSE can be detected in GB sheep |
Strain typing field isolates of sheep scrapie in mice |
| SE1938 |
Strain typing of isolates of natural scrapie: correlations with host PrP genotype and pathological phenotype |
VLA |
2,729,710 |
0 |
1998 |
2006 |
To see if there is any influence of the sheep host on the scrapie strain type |
Strain characterisation in sheep and by mouse bioassay of naturally-occurring scrapie isolates from sheep carrying different genotypes on the same farms |
| SE1948 |
Studies on murine scrapie strains re-isolated in sheep |
MRI |
2,723,006 |
318,140 |
2001 |
2010 |
To characterise the TSE induced by mouse strains in sheep |
Challenges of sheep with murine scrapie strains |
| SE2013 |
Cell free conversion of designed prion proteins to distinguish TSE strains |
Roslin Institute, Compton |
586,237 |
0 |
2007 |
2010 |
To develop a novel rapid strain typing method |
Cell free conversion studies using a range of TSE strains and PrP substrates |
| Infectivity assays and the infectious agent |
| SE1439 |
The relative transmissibility of sheep and cattle BSE to humans |
NPU, Roslin Institute |
1,153,100 |
123,400 |
2001 |
2006 |
To provide information on the relative species barrier to humans and bovines for BSE and scrapie |
Challenge of transgenic mice, which carry bovine or human prion protein genes, with sheep scrapie, sheep passaged BSE, and cattle BSE |
| SE1441 |
The relative transmissibility of atypical forms of scrapie to humans |
NPU, Roslin Institute |
700,685 |
125,370 |
2006 |
2010 |
To provide information on the relative species barrier to humans and bovines for atypical scrapie |
Challenge of transgenic mice, which carry bovine or human prion protein genes, with atypical scrapie, sheep BSE and classical scrapie |
| SE1753 |
Preparation of more sensitive bioassay models for the improved detection, differentiation and diagnosis of BSE |
VLA |
2,029,615 |
199,635 |
1999 |
2008 |
To provide more rapid mouse bioassay methods |
Production of transgenic mice expressing cattle, kudu, and ovine ARR and AHQ PrP genes |
| SE1790 |
Is PrP a reliable marker for infectivity? |
NPU, Roslin Institute |
315,004 |
102,280 |
2004 |
2008 |
To investigate the validity of PrPSc as a marker for TSE infectivity |
Studies using a mouse model where levels of PrPSc and infectivity do not correlate |
| SE2001 |
Application of the Transient Scrapie Cell Assay (TraSCA) for in vitro detection of ovine and bovine prions |
University Hospital Zurich |
194,429 |
64,809 |
2005 |
2008 |
To improve cell culture infectivity assays |
Introduction of murine and ovine cell lines stably expressing tagged ovine and bovine prion proteins |
| SE2002 |
Development of Neural Stem Cell models for the detection and characterisation of ovine and bovine TSE agents |
CNRS, France |
280,830 |
93,467 |
2005 |
2009 |
To improve cell culture infectivity assays |
Adaptation of the neural stem cell model to the detection of ovine and bovine prions |
| SE2003 |
Ex vivo cell culture methods for the detection and characterisation of ovine and bovine TSEs |
VLA |
402,368 |
131,289 |
2006 |
2008 |
To adapt existing cell lines for detecting the infectivity of BSE and scrapie |
Further development of existing cell lines permissive to ovine scrapie and bovine BSE |
| SE2007 |
The relationship between TSE test kit outputs and infectivity levels |
VLA |
537,214 |
121,400 |
2006 |
2009 |
To validate the use of diagnostic tests for measuring infectivity |
Comparison of the results of diagnostic tests for TSEs in sheep with infectivity bioassays in ovinised Tg338 transgenic mice |
| SE2012 |
Infectivity associated to PrP aggregate fractions |
CEA, France |
77,906 |
0 |
2007 |
2009 |
To identify which scrapie fractions contain TSE infectivity |
Titration in mice of fractions (obtained by different combinations of various biochemical protocols) of classical and atypical scrapie |
